diff --git a/scientific-skills/tiledbvcf/SKILL.md b/scientific-skills/tiledbvcf/SKILL.md
index 5a9e652..8391795 100644
--- a/scientific-skills/tiledbvcf/SKILL.md
+++ b/scientific-skills/tiledbvcf/SKILL.md
@@ -63,7 +63,10 @@ import tiledbvcf
 ds = tiledbvcf.Dataset(uri="my_dataset", mode="w",
                       cfg=tiledbvcf.ReadConfig(memory_budget=1024))
 
-# Ingest VCF files (can be run incrementally)
+# Ingest VCF files (must be single-sample with indexes)
+# Requirements:
+# - VCFs must be single-sample (not multi-sample)
+# - Must have indexes: .csi (bcftools) or .tbi (tabix)
 ds.ingest_samples(["sample1.vcf.gz", "sample2.vcf.gz"])
 ```
 
@@ -100,6 +103,10 @@ ds.export(
 
 Create TileDB-VCF datasets and incrementally ingest variant data from multiple VCF/BCF files. This is appropriate for building population genomics databases and cohort studies.
 
+**Requirements:**
+- **Single-sample VCFs only**: Multi-sample VCFs are not supported
+- **Index files required**: VCF/BCF files must have indexes (.csi or .tbi)
+
 **Common operations:**
 - Create new datasets with optimized array schemas
 - Ingest single or multiple VCF/BCF files in parallel
diff --git a/scientific-skills/tiledbvcf/references/ingestion.md b/scientific-skills/tiledbvcf/references/ingestion.md
index b663165..32eba8a 100644
--- a/scientific-skills/tiledbvcf/references/ingestion.md
+++ b/scientific-skills/tiledbvcf/references/ingestion.md
@@ -2,6 +2,22 @@
 
 Complete guide to creating TileDB-VCF datasets and ingesting VCF/BCF files with optimal performance and reliability.
 
+## Important Requirements
+
+**Before ingesting VCF files, ensure they meet these requirements:**
+
+- **Single-sample VCFs only**: Multi-sample VCFs are not supported by TileDB-VCF
+- **Index files required**: All VCF/BCF files must have corresponding index files:
+  - `.csi` files (created with `bcftools index`)
+  - `.tbi` files (created with `tabix`)
+
+```bash
+# Create indexes if they don't exist
+bcftools index sample.vcf.gz        # Creates sample.vcf.gz.csi
+# OR
+tabix -p vcf sample.vcf.gz          # Creates sample.vcf.gz.tbi
+```
+
 ## Dataset Creation
 
 ### Basic Dataset Creation