Support for Ginkgo Cloud Lab

scientific-skills/ginkgo-cloud-lab/references/fluorescent-pixel-art-generation.md

@@ -0,0 +1,87 @@
+# Fluorescent Pixel Art Generation
+
+**URL:** https://cloud.ginkgo.bio/protocols/fluorescent-pixel-art-generation
+**Status:** Beta
+**Price:** $25/plate
+**Turnaround:** 5-7 days
+
+## Overview
+
+Transforms a digital image into a living, fluorescent bacterial artwork printed on an agar omni-tray. Customers submit a pixel art design and colors are mapped to distinct fluorescent E. coli strains. Overnight cultures are prepared from frozen glycerol stocks, diluted, and dispensed onto selective LB-chloramphenicol agar plates via Echo acoustic liquid handling at 50 nL per spot. Plates are incubated at 30 deg C for 16 hours, followed by 4 deg C for 12 hours to stabilize colony morphology and fluorescence. High-resolution photographs are captured under UV illumination and delivered digitally.
+
+## Input
+
+- **Image file:** `.png` or `.svg` format
+- **Resolution:** 48x48 to 96x96 pixels
+- **Color mapping:** Match image colors to the fluorescent strain palette
+- **Orientation:** Confirm plate orientation and multi-plate designs (identical vs. distinct)
+
+## Available Fluorescent E. coli Strains (11 colors)
+
+| Strain/Protein | Color |
+|---|---|
+| sfGFP | Green |
+| mRFP | Red |
+| mKO2 | Orange |
+| Venus | Yellow |
+| Azurite | Blue |
+| mClover3 | Bright Green |
+| mJuniper | Dark Green |
+| mTurquoise2 | Cyan |
+| Electra2 | Electric Blue |
+| mWasabi | Light Green |
+| mScarlet-I | Scarlet |
+
+## Output
+
+- **Digital delivery:** High-resolution UV images in TIFF/JPEG format
+- **Optional add-ons:** Framed archival prints
+
+## Automated Workflow
+
+### Phase 1 - Source Plate Preparation
+
+1. Shake source plate
+2. Centrifuge source plate
+3. Peel source plate seal
+
+### Phase 2 - Acoustic Dispensing (per destination plate)
+
+1. Peel destination seal
+2. Echo hit-pick dispensing (50 nL per spot)
+3. Seal destination plate
+4. Shake destination plate
+5. Centrifuge destination
+6. Store destination at 30 deg C (16 hr incubation)
+
+### Phase 3 - Source Storage
+
+1. Seal source plate
+2. Store source plate
+
+### Post-Processing
+
+1. Transfer to 4 deg C for 12 hours (fluorescence stabilization)
+2. UV illumination photography
+3. Image processing and delivery
+
+## Ordering
+
+- **Number of Plates:** configurable
+- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP, PNG, JPG, GIF, SVG, WEBP
+- **Additional Details:** free-text field for special requirements
+
+## Certification Milestones
+
+- Dry Run Complete
+- Wet Run Complete
+- Biovalidation Complete
+- App Note Complete
+
+## Use Cases
+
+- Educational outreach and demonstrations
+- Unique scientific art and gifts
+- Conference displays and promotional materials
+- Lab team celebrations
+- Visualizing biological art concepts