# Cell Free Protein Expression Validation

**URL:** https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation
**Status:** Ginkgo Certified
**Price:** $39/sample (default: $936 for 8 proteins x 3 replicates = 24 samples)
**Turnaround:** 5-10 days

## Overview

Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.

## Input

- **DNA sequence** in `.fasta` format
- Sequences up to 1800 bp supported

## Output

- **Expression Confirmation:** Verification of target protein at expected molecular weight
- **Baseline Titer:** Initial quantitative yield measurement (mg/L)
- **Initial Purity:** Percentage of target protein vs. impurities, delivered with virtual gel images

## Automated Workflow

### Phase 1 - CFPS Reaction Setup & Incubation

1. Retrieve plates
2. Stamp DNA templates
3. Seal plate
4. Incubate shaking at 30 deg C

### Phase 2 - Quantification Prep

1. Dispense PBS diluent
2. Seal plate
3. Store at 4 deg C

### Phase 3 - LabChip Quantification

1. Unseal plate
2. LabChip quantification
3. Seal plate
4. Store at 4 deg C

## Protocol Parameters

- Payloads & Reagents
- Bravo Stamp
- HiG Centrifuge
- Incubation & Storage

## Ordering

- **Number of Proteins:** configurable
- **Number of Replicates:** configurable
- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
- **Additional Details:** free-text field for special requirements

## Certification Milestones

- Dry Run Complete
- Wet Run Complete
- Biovalidation Complete
- App Note Complete

## Use Cases

- Screening candidate protein sequences for expressibility
- Go/no-go decisions before investing in optimization
- Evaluating novel constructs in a cell-free system
- Comparing expression levels across sequence variants