claude-scientific-skills/scientific-skills/ginkgo-cloud-lab/references/fluorescent-pixel-art-generation.md

Fluorescent Pixel Art Generation

URL: https://cloud.ginkgo.bio/protocols/fluorescent-pixel-art-generation Status: Beta Price: $25/plate Turnaround: 5-7 days

Overview

Transforms a digital image into a living, fluorescent bacterial artwork printed on an agar omni-tray. Customers submit a pixel art design and colors are mapped to distinct fluorescent E. coli strains. Overnight cultures are prepared from frozen glycerol stocks, diluted, and dispensed onto selective LB-chloramphenicol agar plates via Echo acoustic liquid handling at 50 nL per spot. Plates are incubated at 30 deg C for 16 hours, followed by 4 deg C for 12 hours to stabilize colony morphology and fluorescence. High-resolution photographs are captured under UV illumination and delivered digitally.

Input

• Image file: .png or .svg format
• Resolution: 48x48 to 96x96 pixels
• Color mapping: Match image colors to the fluorescent strain palette
• Orientation: Confirm plate orientation and multi-plate designs (identical vs. distinct)

Available Fluorescent E. coli Strains (11 colors)

Strain/Protein	Color
sfGFP	Green
mRFP	Red
mKO2	Orange
Venus	Yellow
Azurite	Blue
mClover3	Bright Green
mJuniper	Dark Green
mTurquoise2	Cyan
Electra2	Electric Blue
mWasabi	Light Green
mScarlet-I	Scarlet

Output

• Digital delivery: High-resolution UV images in TIFF/JPEG format
• Optional add-ons: Framed archival prints

Automated Workflow

Phase 1 - Source Plate Preparation

1. Shake source plate
2. Centrifuge source plate
3. Peel source plate seal

Phase 2 - Acoustic Dispensing (per destination plate)

1. Peel destination seal
2. Echo hit-pick dispensing (50 nL per spot)
3. Seal destination plate
4. Shake destination plate
5. Centrifuge destination
6. Store destination at 30 deg C (16 hr incubation)

Phase 3 - Source Storage

1. Seal source plate
2. Store source plate

Post-Processing

1. Transfer to 4 deg C for 12 hours (fluorescence stabilization)
2. UV illumination photography
3. Image processing and delivery

Ordering

• Number of Plates: configurable
• File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP, PNG, JPG, GIF, SVG, WEBP
• Additional Details: free-text field for special requirements

Certification Milestones

• Dry Run Complete
• Wet Run Complete
• Biovalidation Complete
• App Note Complete

Use Cases

• Educational outreach and demonstrations
• Unique scientific art and gifts
• Conference displays and promotional materials
• Lab team celebrations
• Visualizing biological art concepts