Cell Free Protein Expression Validation

URL: https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation Status: Ginkgo Certified Price: $39/sample (default: $936 for 8 proteins x 3 replicates = 24 samples) Turnaround: 5-10 days

Overview

Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.

Input

DNA sequence in .fasta format
Sequences up to 1800 bp supported

Output

Expression Confirmation: Verification of target protein at expected molecular weight
Baseline Titer: Initial quantitative yield measurement (mg/L)
Initial Purity: Percentage of target protein vs. impurities, delivered with virtual gel images

Automated Workflow

Phase 1 - CFPS Reaction Setup & Incubation

Retrieve plates
Stamp DNA templates
Seal plate
Incubate shaking at 30 deg C

Phase 2 - Quantification Prep

Dispense PBS diluent
Seal plate
Store at 4 deg C

Phase 3 - LabChip Quantification

Unseal plate
LabChip quantification
Seal plate
Store at 4 deg C

Protocol Parameters

Payloads & Reagents
Bravo Stamp
HiG Centrifuge
Incubation & Storage

Ordering

Number of Proteins: configurable
Number of Replicates: configurable
File Upload: CSV, Excel, FASTA, TXT, PDF, ZIP
Additional Details: free-text field for special requirements

Certification Milestones

Dry Run Complete
Wet Run Complete
Biovalidation Complete
App Note Complete

Use Cases

Screening candidate protein sequences for expressibility
Go/no-go decisions before investing in optimization
Evaluating novel constructs in a cell-free system
Comparing expression levels across sequence variants

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