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88 lines
2.6 KiB
Markdown
88 lines
2.6 KiB
Markdown
# Fluorescent Pixel Art Generation
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**URL:** https://cloud.ginkgo.bio/protocols/fluorescent-pixel-art-generation
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**Status:** Beta
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**Price:** $25/plate
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**Turnaround:** 5-7 days
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## Overview
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Transforms a digital image into a living, fluorescent bacterial artwork printed on an agar omni-tray. Customers submit a pixel art design and colors are mapped to distinct fluorescent E. coli strains. Overnight cultures are prepared from frozen glycerol stocks, diluted, and dispensed onto selective LB-chloramphenicol agar plates via Echo acoustic liquid handling at 50 nL per spot. Plates are incubated at 30 deg C for 16 hours, followed by 4 deg C for 12 hours to stabilize colony morphology and fluorescence. High-resolution photographs are captured under UV illumination and delivered digitally.
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## Input
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- **Image file:** `.png` or `.svg` format
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- **Resolution:** 48x48 to 96x96 pixels
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- **Color mapping:** Match image colors to the fluorescent strain palette
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- **Orientation:** Confirm plate orientation and multi-plate designs (identical vs. distinct)
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## Available Fluorescent E. coli Strains (11 colors)
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| Strain/Protein | Color |
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|---|---|
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| sfGFP | Green |
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| mRFP | Red |
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| mKO2 | Orange |
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| Venus | Yellow |
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| Azurite | Blue |
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| mClover3 | Bright Green |
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| mJuniper | Dark Green |
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| mTurquoise2 | Cyan |
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| Electra2 | Electric Blue |
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| mWasabi | Light Green |
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| mScarlet-I | Scarlet |
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## Output
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- **Digital delivery:** High-resolution UV images in TIFF/JPEG format
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- **Optional add-ons:** Framed archival prints
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## Automated Workflow
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### Phase 1 - Source Plate Preparation
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1. Shake source plate
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2. Centrifuge source plate
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3. Peel source plate seal
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### Phase 2 - Acoustic Dispensing (per destination plate)
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1. Peel destination seal
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2. Echo hit-pick dispensing (50 nL per spot)
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3. Seal destination plate
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4. Shake destination plate
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5. Centrifuge destination
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6. Store destination at 30 deg C (16 hr incubation)
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### Phase 3 - Source Storage
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1. Seal source plate
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2. Store source plate
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### Post-Processing
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1. Transfer to 4 deg C for 12 hours (fluorescence stabilization)
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2. UV illumination photography
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3. Image processing and delivery
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## Ordering
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- **Number of Plates:** configurable
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- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP, PNG, JPG, GIF, SVG, WEBP
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- **Additional Details:** free-text field for special requirements
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## Certification Milestones
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- Dry Run Complete
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- Wet Run Complete
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- Biovalidation Complete
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- App Note Complete
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## Use Cases
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- Educational outreach and demonstrations
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- Unique scientific art and gifts
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- Conference displays and promotional materials
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- Lab team celebrations
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- Visualizing biological art concepts
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