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72 lines
2.0 KiB
Markdown
72 lines
2.0 KiB
Markdown
# Cell Free Protein Expression Validation
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**URL:** https://cloud.ginkgo.bio/protocols/cell-free-protein-expression-validation
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**Status:** Ginkgo Certified
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**Price:** $39/sample (default: $936 for 8 proteins x 3 replicates = 24 samples)
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**Turnaround:** 5-10 days
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## Overview
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Fastest path from a protein sequence to a quantitative go/no-go readout on expression. Uses a proprietary reconstituted E. coli transcription-translation (cell-free protein synthesis, CFPS) system. Reactions complete in 4-16 hours. Designed for early-stage screening, novel construct evaluation, and rapid triage of candidate sequences before committing resources to downstream optimization or purification.
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## Input
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- **DNA sequence** in `.fasta` format
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- Sequences up to 1800 bp supported
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## Output
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- **Expression Confirmation:** Verification of target protein at expected molecular weight
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- **Baseline Titer:** Initial quantitative yield measurement (mg/L)
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- **Initial Purity:** Percentage of target protein vs. impurities, delivered with virtual gel images
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## Automated Workflow
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### Phase 1 - CFPS Reaction Setup & Incubation
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1. Retrieve plates
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2. Stamp DNA templates
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3. Seal plate
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4. Incubate shaking at 30 deg C
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### Phase 2 - Quantification Prep
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1. Dispense PBS diluent
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2. Seal plate
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3. Store at 4 deg C
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### Phase 3 - LabChip Quantification
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1. Unseal plate
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2. LabChip quantification
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3. Seal plate
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4. Store at 4 deg C
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## Protocol Parameters
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- Payloads & Reagents
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- Bravo Stamp
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- HiG Centrifuge
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- Incubation & Storage
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## Ordering
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- **Number of Proteins:** configurable
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- **Number of Replicates:** configurable
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- **File Upload:** CSV, Excel, FASTA, TXT, PDF, ZIP
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- **Additional Details:** free-text field for special requirements
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## Certification Milestones
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- Dry Run Complete
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- Wet Run Complete
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- Biovalidation Complete
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- App Note Complete
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## Use Cases
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- Screening candidate protein sequences for expressibility
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- Go/no-go decisions before investing in optimization
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- Evaluating novel constructs in a cell-free system
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- Comparing expression levels across sequence variants
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